Ligation Technology
- Simply clone protein of interest as an intein fusion
- Express fusion protein in host of choice
- Affinity purify fusion protein from lysates using commercially available reagents
- Treat fusion protein with aqueous hydrazine
- Enzyme free liberation of target protein (folded) with unique C-terminal hydrazide functionality
- No extra amino acids appended to target sequence
- Recombinant C-terminal hydrazide protein can be produced in folded form
- Treat Recombinant hydrazide protein with pyruvoyl-PEG reagent(s) under aqueous conditions.
- Site-specific mono-PEGylated folded protein generated in high yield.
Compatible with disulphide bond containing proteins
This technology is covered by WO 2005014620.
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